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KMID : 0545120110210060567
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Journal of Microbiology and Biotechnology
2011 Volume.21 No. 6 p.567 ~ p.573
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The Phylogenetic Affiliation of an Uncultured Population of Ammonia-Oxidizing Bacteria Harboring Environmental Sequences of amoA Cluster-3
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Hong Jin-Kyung
Cho Jae-Chang
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Abstract
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We investigated the phylogenetic diversity of ammoniaoxidizing bacteria (AOB) in Yellow Sea continental shelf sediment by the cloning and sequencing of PCR-amplified amoA and 16S rRNA genes. Phylogenetic analysis of the amoA-related clones revealed that the diversity of AOB was extremely low at the study site. The majority (92.7%) of amoA clones obtained belonged to a single cluster, environmental amoA cluster-3, the taxonomic position of which was previously unknown. Phylogenetic analysis on AOB-specific 16S rRNA gene sequences also demonstrated a very low diversity. All of the cloned 16S rRNA gene sequences comprised a single phylotype that belonged to the members of uncultured Nitrosospira cluster-1, suggesting that AOB belonging to the uncultured Nitrosospira cluster- 1 could carry amoA sequences of environmental amoA cluster-3.
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KEYWORD
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Ammonia-oxidizing bacteria, amoA, phylogeny
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